It is ‘mitochondrial coupling states’, which includes aspects of uncoupling (e.g. ‘OXPHOS coupling’ might be too close a term to ‘OXPHOS state’.
How proton motive force drives etc full#
100 % coupling is full transfer of energy” I would therefor prefer States of OXPHOS coupling, with coupling defined as linked in terms of energy transfer. personally I am not sure that the term coupling state is actually the best, since this refers to coupling of OXPHOS only (and not energetic coupling in general). So please consider them in a supportive manner.Ĭoupling states: “since this is the key of the paper, I think this has to be defined here. I put them down, because I think that if I have comments, also others will and addressing them will help to improve the manuscript. Some of the comments are, :::: I think, quite fundamental and likely stem from the fact that I am not a mitochondrial bio-energeticist. An impressive piece of work already and good for the community! I have a number of comments, and I hpope they are of use. Herewith my comment to the mitoeagle consensus review.Wow you work fast! The document really is great and I can see that you've put a lot of work into it, so I hope that our very minor comments were of some use.The tendency for mitochondria of specific sizes to be enriched at different centrifugation speeds also has the potential to allow the isolation of specific mitochondrial subpopulations and therefore the analysis of mitochondria from multiple cell lineages within a single tissue.
This possible artefact should be taken into account when planning experiments using isolated mitochondria. The isolation of mitochondria (often achieved through differential centrifugation) can therefore yield a subsample of the mitochondrial types present in a tissue, dependent on isolation protocols utilised (e.g. Mitochondria are also in a constant state of flux due to highly dynamic fission and fusion cycles, and can exist in multiple stages and sizes which may be altered by a range of factors.
Phase 3: : Publication as a preprint: MitoFit Preprints with DOI number, providing widely accepted visible proof that the publication is citable.» MitoEAGLE Task Group States and rates - Discussion Phase 2: Mitochondrial respiratory states and rates: Building blocks of mitochondrial physiology Part 1.: Print version (16) for MiP2017/MitoEAGLE Hradec Kralove CZ.» The protonmotive force and respiratory control - Discussion
Phase 1: The protonmotive force and respiratory control.We hope to contribute to this evolutionary process, with an emphasis on harmonization rather than standardization. In the long run, superior standards will become accepted. Some established researchers in the field may not want to re-consider the use of jargon which has become established despite deficiencies of accuracy and meaning. Quality of research requires quality of communication. We hope to initiate a much broader process of discussion and want to raise the awareness on the importance of a consistent terminology for reporting of scientific data in the field of bioenergetics, mitochondrial physiology and pathology. Our manuscript aims at providing arguments for further debate rather than pushing opinions. Nevertheless, we do not consider scientific progress to be supported by ‘declaration’ statements (other than on ethical or political issues). The global MitoEAGLE network made it possible to collaborate with a large number of coauthors to reach consensus on the present manuscript. The list of coauthors evolved beyond phase 1 in the bottom-up spirit of COST. Manuscript phases and versions - an open-access apporach COST Action MitoEAGLE This manuscript on ‘Mitochondrial respiratory states and rates’ is a position statement in the frame of COST Action CA15203 MitoEAGLE.
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